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Image Search Results
Journal: JCI Insight
Article Title: Endothelial transcriptomic analysis identifies biomarkers of severe and cerebral malaria
doi: 10.1172/jci.insight.172845
Figure Lengend Snippet: HBMEC were incubated with iRBCL at different concentrations (expressed as number of iRBC per cm 2 ), RBCL (8 × 10 6 /cm 2 ), or TNF at the indicated ng/mL concentrations for 24 hours before collection of the culture media and determination of the levels of angiopoietin-2 by ELISA. Results represent the mean of duplicates of 3 independent experiments with SD. Statistical significance was determined by 1-way ANOVA with Dunnett’s multiple-comparison test (**** P < 0.0001).
Article Snippet: Either samples from HBMEC culture media in different conditions or plasma from children in the cohort were used to determine the levels of the different candidate biomarkers using commercial
Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Comparison
Journal: JCI Insight
Article Title: Endothelial transcriptomic analysis identifies biomarkers of severe and cerebral malaria
doi: 10.1172/jci.insight.172845
Figure Lengend Snippet: HBMEC were incubated for the indicated time points with media (black line), TNF (1 μg/mL; gray line), RBCL (8 × 10 6 RBC/cm 2 ; pink line), or P . falciparum –iRBCL (8 × 10 6 iRBC/cm 2 ; purple line). The medium of independent duplicates for each condition was collected at each time point. Levels of candidate biomarker proteins were determined by ELISA. Graphs show the average of the duplicated determinations for each time point.
Article Snippet: Either samples from HBMEC culture media in different conditions or plasma from children in the cohort were used to determine the levels of the different candidate biomarkers using commercial
Techniques: Incubation, Biomarker Discovery, Enzyme-linked Immunosorbent Assay
Journal: JCI Insight
Article Title: Endothelial transcriptomic analysis identifies biomarkers of severe and cerebral malaria
doi: 10.1172/jci.insight.172845
Figure Lengend Snippet: Comparison between the levels of the candidate biomarkers in samples from randomly selected patients with UM ( n = 17) and all available patients with CM ( n = 23) by ELISA. Mean level (red line). Statistical significance was determined by Mann-Whitney U test (** P < 0.01; **** P < 0.0001).
Article Snippet: Either samples from HBMEC culture media in different conditions or plasma from children in the cohort were used to determine the levels of the different candidate biomarkers using commercial
Techniques: Comparison, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY
Journal: JCI Insight
Article Title: Endothelial transcriptomic analysis identifies biomarkers of severe and cerebral malaria
doi: 10.1172/jci.insight.172845
Figure Lengend Snippet: Levels of the indicated biomarkers were determined in the plasma of children with UM ( n = 128) or SM ( n = 136) malaria by ELISA. Mean level (red line). Statistical significance was determined by Mann-Whitney U test (*** P < 0.001; **** P < 0.0001). AUC with 95% CI and P value are shown.
Article Snippet: Either samples from HBMEC culture media in different conditions or plasma from children in the cohort were used to determine the levels of the different candidate biomarkers using commercial
Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY
Journal: JCI Insight
Article Title: Endothelial transcriptomic analysis identifies biomarkers of severe and cerebral malaria
doi: 10.1172/jci.insight.172845
Figure Lengend Snippet: Levels of the indicated biomarkers were determined in the plasma of children with severe noncerebral (SM; n =113) or cerebral (CM; n = 23) malaria by ELISA. Mean level (red line). Relative units (RU) were calculated for each determination dividing by the average value of the control (SM) group. The highest of ANGPTL4 and INHBE values expressed in RU were selected and compared between SM and CM groups. Statistical significance was determined by Mann-Whitney U test (** P < 0.01; **** P < 0.0001). AUC with 95% CI and P value are shown.
Article Snippet: Either samples from HBMEC culture media in different conditions or plasma from children in the cohort were used to determine the levels of the different candidate biomarkers using commercial
Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control, MANN-WHITNEY